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人結(jié)腸癌細(xì)胞(SW620)

￥800.00元

細(xì)胞貨號(hào)： CL0305

細(xì)胞種屬： 人源細(xì)胞系

細(xì)胞別名： SW-620; SW 620; SW.620

發(fā)貨規(guī)格： 1×10?

供體信息： 51歲白人男性

腫瘤類型： 結(jié)直腸腺癌

組織來(lái)源： 大腸

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背景信息

種屬：人源細(xì)胞系
組織來(lái)源：大腸
腫瘤類型：結(jié)直腸腺癌
供體信息： 51歲白人男性
背景描述： SW-620細(xì)胞系來(lái)自一名患有Dukes-C型結(jié)直腸癌的51歲男性的大腸，是結(jié)直腸癌的關(guān)鍵研究模型，特別是用于癌癥生物標(biāo)志物、化療和轉(zhuǎn)移性癌細(xì)胞的研究。SW-620細(xì)胞是研究細(xì)胞凋亡和抗失巢凋亡機(jī)制的關(guān)鍵，失巢凋亡是一種對(duì)防止轉(zhuǎn)移至關(guān)重要的程序性細(xì)胞死亡形式。利用SW-620結(jié)腸癌細(xì)胞的研究已經(jīng)深入到蛋白質(zhì)組分析中，以了解不同條件下（如缺氧）的蛋白質(zhì)組變化。低氧SW620細(xì)胞表現(xiàn)出特定的蛋白質(zhì)組適應(yīng)，這有助于化療耐藥性。

培養(yǎng)建議

其他信息

蛋白表達(dá)： keratin
同工酶： ES-D, 1；G6PD, B；PEP-D, 1；PGD, A；PGM1, 2；PGM3, 1
基因表達(dá)： carcinoembryonic antigen (CEA) 0.15 ng/106 cells/10 days; transforming growth factor alpha; matrilysin; CSAp negative; and colon antigen 3 negative; keratin positive by immunoperoxidase staining; c-myc; K-ras; H-ras; N-ras; Myb; sis; fos
致癌基因： myc+; myb+; ras+; fos+; sis+; p53+; abl-; ros-; src-
致瘤性： Yes, in nude mice。(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
抗原表達(dá)： Blood type A; Rh+
突變信息： There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.
核型： modal number = 50; range = 45 to 53. The stemline chromosome number is hyperdiploid with the 2S component occurring at 12% and 11 marker chromosomes were common to S metaphases. One of the 11 markers [7 (7p; 11p)] was disomic., M1 was probably an HSR but the origin of the chromosome could not be identified.

人結(jié)腸癌細(xì)胞

Catalog No CL0305

Product information

蛋白表達(dá)： keratin
同工酶： ES-D, 1；G6PD, B；PEP-D, 1；PGD, A；PGM1, 2；PGM3, 1
基因表達(dá)： carcinoembryonic antigen (CEA) 0.15 ng/106 cells/10 days; transforming growth factor alpha; matrilysin; CSAp negative; and colon antigen 3 negative; keratin positive by immunoperoxidase staining; c-myc; K-ras; H-ras; N-ras; Myb; sis; fos
致癌基因： myc+; myb+; ras+; fos+; sis+; p53+; abl-; ros-; src-
致瘤性： Yes, in nude mice。(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
抗原表達(dá)： Blood type A; Rh+
突變信息： There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.
核型： modal number = 50; range = 45 to 53. The stemline chromosome number is hyperdiploid with the 2S component occurring at 12% and 11 marker chromosomes were common to S metaphases. One of the 11 markers [7 (7p; 11p)] was disomic., M1 was probably an HSR but the origin of the chromosome could not be identified.

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