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人皮膚惡性黑色素瘤細(xì)胞(SK-MEL-28)

￥800.00元

細(xì)胞貨號(hào)： CL0284

細(xì)胞種屬： 人源細(xì)胞系

細(xì)胞別名： SK-Mel-28; SK.MEL.28; SK-MEL 28; SK MEL-28; SK MEL 28; SK Mel 28; SKMel-28; SKMEL-28; SK-MEL28; SK-Mel28; SK Mel28; SKMEL28; SKMel28; SKmel28; SKML-28; SK28; AU-Mel; P-36; P36

發(fā)貨規(guī)格： 1×10?

供體信息： 51歲男性

腫瘤類型： 皮膚黑色素瘤

組織來源： 皮膚

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背景信息

種屬：人源細(xì)胞系
組織來源：皮膚
腫瘤類型：皮膚黑色素瘤
供體信息： 51歲男性
背景描述：該細(xì)胞系是由 T.Takahashi 及其同事從一名種族不明的 51 歲男性的腋窩淋巴結(jié)中建立的，他們?cè)谝幌盗泻谏亓鱿抵蟹蛛x出該細(xì)胞系。

培養(yǎng)建議

生長(zhǎng)形態(tài)：多角形
生長(zhǎng)特性：貼壁
培養(yǎng)條件： RPMI-1640+10%FBS+1%P/S
凍存條件： 90%FBS+10%DMSO
血清使用等級(jí)：標(biāo)準(zhǔn)
傳代方式：胰酶消化
倍增時(shí)間： 27h
傳代比例：胰酶消化
換液頻率：每周2~3次
生物安全等級(jí)： 1
培養(yǎng)注意事項(xiàng)：

其他信息

蛋白表達(dá)： p53 positive
同工酶： PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B
致瘤性： Yes, in nude mice. Forms malignant melanoma (large round cell type)
抗原表達(dá)： Blood Type A; Rh+; HLA A11, A26, B40, DRw4
突變信息： BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot), N-Ras wt
核型： modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell.

人皮膚惡性黑色素瘤細(xì)胞

Catalog No CL0284

Product information

蛋白表達(dá)： p53 positive
同工酶： PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B
致瘤性： Yes, in nude mice. Forms malignant melanoma (large round cell type)
抗原表達(dá)： Blood Type A; Rh+; HLA A11, A26, B40, DRw4
突變信息： BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot), N-Ras wt
核型： modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell.

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