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人乳腺癌細(xì)胞(MDA-MB-231)

￥800.00元

細(xì)胞貨號： CL0208

細(xì)胞種屬： 人源細(xì)胞系

細(xì)胞別名： MDA_MB_231;MDA-MB231;MDA.MB.231;MDAMB231；MDA-MB231;MDAMB-231;MDAMB231;MDA-231;MDA-231P;MDA231;MDA231-BRE;MB231;MD Anderson-Metastatic Breast-231

發(fā)貨規(guī)格： 1×10?

供體信息： 51歲白人女性

腫瘤類型： 乳腺癌

組織來源： 乳腺

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背景信息

種屬：人源細(xì)胞系
組織來源：乳腺
腫瘤類型：乳腺癌
供體信息： 51歲白人女性
背景描述：成立于1973年，由一名51歲浸潤性乳腺癌女性化療后胸腔積液而成;廣泛使用和廣泛表征的細(xì)胞系;三陰性，并被描述為在裸鼠中具有致瘤性

培養(yǎng)建議

生長形態(tài)：上皮細(xì)胞樣
生長特性：貼壁
培養(yǎng)條件： DMEM/F12+5%馬血清+10ug/ml胰島素+20ng/ml表皮生長因子+100ng/ml霍亂毒素+0.5ug/mL氫化可的松
凍存條件： 90%FBS+10%DMSO
血清使用等級：標(biāo)準(zhǔn)
傳代方式：胰酶消化
倍增時間： 25~30h
傳代比例：胰酶消化
換液頻率：每周2~3次
生物安全等級： 1
培養(yǎng)注意事項(xiàng)：

其他信息

蛋白表達(dá)： Epidermal growth factor(EGF),expressed;transforming growth factoral pha(TGFalpha),expressed
受體表達(dá)： Epidermal growth factor (EGF), expressed; transforming growth factor alpha (TGF alpha), expressed
同工酶： AK-1,1；ES-D,1；G6PD,B；GLO-I,2；Me-2,1-2；PGM1,1-2；PGM3,1
致癌基因： wnt7b
致瘤性： Yes, in ALS treated BALB/c mice, forms poorly differentiated adenocarcinoma (grade III)；Yes, in nude mice, forms poorly differentiated adenocarcinoma (grade III)
核型： The cell line is aneuploid female (modal number = 64, range = 52 to 68), with chromosome counts in the near-triploid range. Normal chromosomes N8 and N15 were absent. Eleven stable rearranged marker chromosomes are noted as well as unassignable chromosomes in addition to the majority of autosomes that are trisomic. Many of the marker chromosomes are identical to those shown in the karyotype reported by K.L. Satya-Prakash, et al.

人乳腺癌細(xì)胞

Catalog No CL0208

Product information

蛋白表達(dá)： Epidermal growth factor(EGF),expressed;transforming growth factoral pha(TGFalpha),expressed
受體表達(dá)： Epidermal growth factor (EGF), expressed; transforming growth factor alpha (TGF alpha), expressed
同工酶： AK-1,1；ES-D,1；G6PD,B；GLO-I,2；Me-2,1-2；PGM1,1-2；PGM3,1
致癌基因： wnt7b
致瘤性： Yes, in ALS treated BALB/c mice, forms poorly differentiated adenocarcinoma (grade III)；Yes, in nude mice, forms poorly differentiated adenocarcinoma (grade III)
核型： The cell line is aneuploid female (modal number = 64, range = 52 to 68), with chromosome counts in the near-triploid range. Normal chromosomes N8 and N15 were absent. Eleven stable rearranged marker chromosomes are noted as well as unassignable chromosomes in addition to the majority of autosomes that are trisomic. Many of the marker chromosomes are identical to those shown in the karyotype reported by K.L. Satya-Prakash, et al.

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