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Chk1 Monoclonal Antibody

商品貨號(hào)： PLA004729

適應(yīng) 性： 人,小鼠

￥600元

規(guī)格：

40μL 100μL 200μL

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MSDS

說明書

商品描述

發(fā)貨日期： 7
基因名稱： CHK1
蛋白名稱： Serine/threonine-protein kinase Chk1
Human_gene_id： 1111
Human_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1111
Human_swiss_prot_no： O14757
Human_swiss_link： http://www.uniprot.org/uniprotkb/O14757/entry
Mouse_gene_id： 12649
Mouse_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12649
Mouse_swiss_prot_no： O35280
Mouse_swiss_link： http://www.uniprot.org/uniprot/O35280
特異性： Chk1 Monoclonal Antibody detects endogenous levels of Chk1 protein.
組成： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
來源： Monoclonal, Mouse
稀釋： WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
純化工藝： Affinity purification
濃度： 1 mg/ml
儲(chǔ)存： -15°C to -25°C/1 year(Do not lower than -25°C)
說明書： 1
Msds： MSDS_Antibody.pdf
其他名稱： CHEK1; CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1
信號(hào)通路： Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;p53;
功能： catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory region (AIR) inhibits the activity of the kinase domain.,function:Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.,PTM:Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. May also be phosphorylated at Ser-280 by AKT1/PKB, which may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, which results in decreased activity.,PTM:Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.,subunit:Interacts with BRCA1, CLSPN, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta.,tissue specificity:Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.,
相關(guān)產(chǎn)品： RS0001,RS0002,YM3028,YM3029
細(xì)胞定位： Nucleus . Chromosome . Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nuclear export is mediated at least in part by XPO1/CRM1 (PubMed:12676962). Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B (PubMed:15311285). Proteolytic cleavage at the C-terminus by SPRTN promotes removal from chromatin (PubMed:31316063). .
組織表達(dá)： Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.
科研貨號(hào)： PLA004729

Chk1 Monoclonal Antibody

Catalog No PLA004729

Product information

發(fā)貨日期： 7
基因名稱： CHK1
蛋白名稱： Serine/threonine-protein kinase Chk1
Human_gene_id： 1111
Human_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1111
Human_swiss_prot_no： O14757
Human_swiss_link： http://www.uniprot.org/uniprotkb/O14757/entry
Mouse_gene_id： 12649
Mouse_gene_link： http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12649
Mouse_swiss_prot_no： O35280
Mouse_swiss_link： http://www.uniprot.org/uniprot/O35280
特異性： Chk1 Monoclonal Antibody detects endogenous levels of Chk1 protein.
組成： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
來源： Monoclonal, Mouse
稀釋： WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
純化工藝： Affinity purification
濃度： 1 mg/ml
儲(chǔ)存： -15°C to -25°C/1 year(Do not lower than -25°C)
說明書： 1
Msds： MSDS_Antibody.pdf
其他名稱： CHEK1; CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1
信號(hào)通路： Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;p53;
功能： catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory region (AIR) inhibits the activity of the kinase domain.,function:Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.,PTM:Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. May also be phosphorylated at Ser-280 by AKT1/PKB, which may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, which results in decreased activity.,PTM:Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.,subunit:Interacts with BRCA1, CLSPN, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta.,tissue specificity:Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.,
相關(guān)產(chǎn)品： RS0001,RS0002,YM3028,YM3029
細(xì)胞定位： Nucleus . Chromosome . Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nuclear export is mediated at least in part by XPO1/CRM1 (PubMed:12676962). Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B (PubMed:15311285). Proteolytic cleavage at the C-terminus by SPRTN promotes removal from chromatin (PubMed:31316063). .
組織表達(dá)： Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.
科研貨號(hào)： PLA004729

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